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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Bone marrow derived mesenchymal stem cells ameliorate inflammatory response in an in vitro model of familial hemophagocytic lymphohistiocytosis 2

Fig. 3

Functional validation of selected engineered NK92 clones verified by qPCR and ELISA. Targeted clones did not show an induction of PRF1 mRNA expression following stimulation with PMA and ionomycin (a). Likewise, perforin protein analysis by ELISA indicated a similar profile. Upon stimulation, control NK92 cells (parental cells) secreted several fold of perforin. However, selected clones did not exhibit any significant change (b). Mean values of biological replicates shown, error bars show standard deviation. (+) indicates stimulated, (-) indicates unstimulated cells. *p < 0.05, Student’s t test; n = 6

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Stem Cell Research & Therapy

ISSN: 1757-6512