Fig. 3

a Schematic representation of different layers of differentiation observed using confocal microscopy. Different layers of neurogenic (b), adipogenic (c) and osteogenic (d) differentiation. Confocal observation at day 22, stained with Hoechst and fluorescein diacetate. For each differentiation, panels represent (from left to right) bottom layer (L1, layer in contact with the coverslide), second (L2), third (L3) and fourth (L4) layer, respectively. Grids below represent position of confocal slice on Z axis. Calibration bars correspond to 50 μm. Neuro neurogenic, Adipo adipogenic, Osteo osteogenic