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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: In vitro osteoblastic differentiation of mesenchymal stem cells generates cell layers with distinct properties

Fig. 5

a Immunostaining of upper and lowest layers in osteogenic differentiation. b Comparison of number of Ca2+ oscillations between upper and lowest layers on different days of osteogenic differentiation. haMSC controls (undifferentiated haMSCs grown in classical MSC culture medium) have significantly more Ca2+ oscillations than differentiated layers (****p < 0.0001, one-way ANOVA with Dunnett’s multiple comparisons test). At day 8, Ca2+ oscillations were significantly lower in number in lowest layer than in upper layers (**p < 0.01, p = 0. 0038, two-way ANOVA with Sidak’s multiple comparisons test). Whiskers represent 95th and 5th percentiles. Ctrl control, tryps lowest layer trypsinized, Coll upper layers dissociated with collagenase I, MSC mesenchymal stem cell

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