Fig. 2
From: Effects of lung and airway epithelial maturation cocktail on the structure of lung bud organoids
Morphological features of lung bud organoids (LBOs) and paddle-racquet lung organoids (PRLOs), and expression of alveolar cells markers. a Representative micrographs of LBOs and PRLOs. Pictures 1–3 are representative micrographs of LBOs at day 38 showing the typical finger-shaped extensions. Scale bars = 200 μm (pictures 1,2) and 100 μm (picture 3). Picture 4 shows the expression of surfactant protein B (SFTPB) by immunofluorescence. Scale bar = 50 μm. b Pictures 5 and 6 show the effect of the treatment with the lung and airway epithelial maturation cocktail (path “C”, see text); Scale bars = 200 μm. c Pictures 7 and 8 are representative micrographs showing the detachment of material from the inner surface of the growing PRLO. Scale bars = 100 μm. d. Pictures 9 and 10 are representative immunofluorescence micrographs showing the expression of SFTPB at the surface of PRLOs. Scale bars = 50 μm. e Expression of surfactant genes (ATII cell markers) and AQP5 (ATI cell marker) by qRT-PCR