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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Role of Notch1 in the arterial specification and angiogenic potential of mouse embryonic stem cell-derived endothelial cells

Fig. 4

Effects of Notch1 activation on expression of arterial and venous EC markers in iICN1-ECs. a Western blotting of ICN1, arterial marker Ephrin B2, and venous marker Coup-TFII in iICN1-ECs treated with or without Dox for indicated time. b, c Immunostaining of Ephrin B2 and Coup-TFII in iICN1-ECs. Expression of Ephrin B2 (green), Coup-TFII (green), and ICN1 (red) probed by immunostaining in iICN1-ECs treated with or without Dox for 3 days. Nuclei stained with DAPI (blue), merged images of the three colors shown. Scale bars: 50 μm. DAPI 4′,6-diamidino-2-phenylindole, Dox doxycycline, GAPDH glyceraldehyde 3-phosphate dehydrogenase, iICN1 inducible intracellular domain of Notch1

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Stem Cell Research & Therapy

ISSN: 1757-6512