Fig. 7

Effects of ICN1-induced arterial specification of ECs on therapeutic angiogenesis in vivo. a Representative images of ischemic hindlimb samples at 3 weeks after immunostaining with anti-CD31 antibody or anti-α-SMA antibody (red). Nuclei counterstained with DAPI (blue). b, c Quantitative analysis of CD31-positive capillaries (b) or α-SMA-positive blood vessels (c) in ischemic hindlimb on day 21. Average values presented as mean ± SD (n = 8). *p < 0.05, **p < 0.01, ***p < 0.001. Scale bar = 100 μm. α-SMA alpha smooth muscle actin, DAPI 4′,6-diamidino-2-phenylindole, Dox doxycycline, HPF high-power field