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Fig. 8 | Stem Cell Research & Therapy

Fig. 8

From: Role of Notch1 in the arterial specification and angiogenic potential of mouse embryonic stem cell-derived endothelial cells

Fig. 8

Effects of ICN1 induction on blood vessel formation mediated by ECs in vivo. iICN1-ECs treated with or without Dox for 3 days, labeled with CMFDA, and followed by transplantation into ischemic hindlimb. Hindlimb tissues on day 7 subjected to immunostaining with anti-CD31 antibody (a) and anti-α-SMA antibody (c) (red), nuclei stained with DAPI (blue). Arrows indicate α-SMA-positive or CD31-positive blood vessels containing CMFDA-positive iICN1-ECs. Scale bar = 100 μm. Numbers of capillaries containing both CMFDA and CD31 double-positive cells (b) and number of blood vessels bearing both CMFDA and α-SMA double-positive cells (d). Results presented as mean ± SD (n = 7). *p < 0.05, **p < 0.01. α-SMA alpha smooth muscle actin, DAPI 4′,6-diamidino-2-phenylindole, Dox doxycycline, EC endothelial cell, iICN1 inducible intracellular domain of Notch1

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