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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Epigenetic modification of mesenchymal stromal cells enhances their suppressive effects on the Th17 responses of cells from rheumatoid arthritis patients

Fig. 3

The effects of epi-MSCs on Th17 cell differentiation. We cultured human PBMCs isolated from three healthy volunteers with untreated, A2V5-treated, A2V10-treated, D100T100-treated, and D100T500-treated human mesenchymal stromal cells (MSCs). Then, we cultured the cells under Th17-polarizing conditions for 48 h. a The proliferation of effector Th17 cells measured using carboxyfluorescein succinimidyl ester (CFSE) staining. We measured the percentages of b IL-17+CD4+ T cells, c IFN-γ+CD4+ T cells, and d CD25highFoxp3+CD4+ T cells by flow cytometry (n = 3). The bars represent the mean ± SD. *P < 0.05, **P < 0.01. A 5-azacitidine, D 5-aza-2′-deoxycytidine, T trichostatin A, V valproic acid

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