Fig. 5
The effects of epi-MSCs on Th17-polarized CD4+ T cells from the SFMCs of RA patients. We cocultured SFMCs isolated from three patients with RA with untreated, A2V5-treated, A2V10-treated, D100T100-treated, and D100T500-treated human mesenchymal stromal cells (MSCs). Then we cultured the cells under Th17-polarizing conditions for 48 h. The SFMCs were stained with anti-CD4-PE-Cy7, anti-CD25-APC, anti-IL-17-PE, and anti-Foxp3-FITC. The CD4+ T cells were gated for further analysis of the percentage of a IL-17+CD4+ T cells and b CD25highFoxp3+CD4+ T cells by flow cytometry. We measured the production of c IL-17, d IL-10, and e IL-2 in the culture supernatants by ELISA (n = 3). The bars represent the mean ± SD. *P < 0.05, **P < 0.0. A 5-azacitidine, D 5-aza-2′-deoxycytidine, T trichostatin A, V valproic acid 1