Fig. 4

Gene expression changes in JMJD6-knockdown ADSCs. a Volcano plot analysis of differential gene expression (log2-fold changes and the corresponding p values for each gene). Red dots represent upregulated genes (n = 3934) and green dots represent downregulated genes (n = 2941). b The heatmap shows the FPKM value changes of the top 10 genes (up and downregulated) related to the cell cycle and cellular movement from control ADSCs and JMJD6-depleted ADSCs. Each colored square represents a biological replicate, n = 3. c qRT-PCR analysis was performed to validate the expression changes of the indicated genes in JMJD6 knockdown ADSCs. The y-axis represents the relative mRNA fold change, which is calculated using the 2^−ΔΔCt formula with β-actin as internal control. Error bars represent the SD. ADSCs adipose-derived mesenchymal stem cells, FPKM fragments per kilobase per million, JMJD6 Jumonji C domain-containing protein 6