Table 1 Primers for quantitative real-time polymerase chain reaction analysis
Gene | Product size (base pairs) | Sequences |
|---|---|---|
MYC | 189 | Forward: 5′-TCCTTTATCACACCCCCTACC-3′ Reverse: 5′-TTAGCCAAATTGTTCCCTGC-3′ |
CCND1 | 94 | Forward: 5′-CCGAGAAGCTGTGCATCTACAC-3′ Reverse: 5′-AGGTTCCACTTGAGCTTGTTCAC-3′ |
AXIN2 | 112 | Forward: 5′-GGCTCCAGAAGATCACAAAG-3′ Reverse: 5′-TATGGAATTTCTTCCCCACA-3′ |
POU5F1 | 115 | Forward: 5′-AGCAAAACCCGGAGGAGT-3′ Reverse: 5′-CCACATCGGCCTGTGTATATC-3′ |
LRP5 | 138 | Forward: 5′-GACCCAGCCCTTTGTTTTGAC-3′ Reverse: 5′-TGTGGACGTTGATGGTATTGGT-3′ |
β-catenin | 77 | Forward: 5′-AAGTGGGTGGTATAGAGGCTCTTG-3′ Reverse: 5′-GATGGCAGGCTCAGTGATGTC-3′ |
ACTB | 108 | Forward: 5′-TTGCTGACAGGATGCAGAAG-3′ Reverse: 5′-TAGAGCCACCAATCCACACA-3′ |