Skip to main content
Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: The legacy effects of electromagnetic fields on bone marrow mesenchymal stem cell self-renewal and multiple differentiation potential

Fig. 3

BMSCs pretreated with EMF exhibited stronger osteogenic differentiation potential. a RUNX2, ALP, OPN, and OCN mRNA levels of two groups analyzed by RT-PCR. GAPDH used as loading control for quantification (n = 3). b Expression of OPN and RUNX2 proteins of both groups determined by western blot analysis. Relative densitometer values quantified by ImageJ software, GAPDH used as internal control (n = 3). c Images of Alizarin Red S staining exhibited plaques of calcified extracellular matrix of both groups. Scale bar = 100 μm. d Semi-quantitative analysis of Alizarin Red S staining among both groups (n = 6). Data shown as mean ± SD. *P < 0.05, **P < 0.01. EMF electromagnetic field

Back to article page