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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: The legacy effects of electromagnetic fields on bone marrow mesenchymal stem cell self-renewal and multiple differentiation potential

Fig. 5

EMF treatment had lasting impact on BMSC adipogenic differentiation potential. a PPARγ2, AP2, and ADIPOQ mRNA levels of two groups detected by RT-PCR. GAPDH served as loading control for quantification (n = 3). b Expression of ADIPOQ and PPARγ2 proteins of both groups determined by western blot analysis. Relative densitometer values quantified by ImageJ software, GAPDH used as internal control (n = 3). c Images of Oil Red O staining showed lipid droplets of both groups. Scale bar = 25 μm. d Semiquantitative analysis of Oil Red O staining among both groups (n = 6). Data shown as mean ± SD. *P < 0.05, **P < 0.01. EMF electromagnetic field

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