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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Single-molecule fluorescence in-situ hybridization reveals that human SHANK3 mRNA expression varies during development and in autism-associated SHANK3 heterozygosity

Fig. 5

SHANK3 mRNA expression in SHANK3+/− male with ASD shows reduction restricted to processes. a Top to bottom: bright-field image, smFISH maximal projections (MIP) and MIP with identified puncta superimposed; both control (left) and SHANK3+/− heterozygous patient (right) hiPSC-derived cortical neurons, 53 DIV. b SHANK3 mRNA puncta per 100 μm2 within cell body of control (n = 16) and SHANK3+/− heterozygous patient (n = 20) hiPSC-derived cortical neurons. c SHANK3 mRNA puncta per 100 μm2 within processes of control (n = 16) and SHANK3+/− heterozygous patient (n = 20) hiPSC-derived cortical neurons. *p = 0.0104 (unpaired t test). Errors bars indicate standard error of mean. SHANK3 SH3 and multiple ankyrin repeat domains 3

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Stem Cell Research & Therapy

ISSN: 1757-6512