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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Establishment of a complex skin structure via layered co-culture of keratinocytes and fibroblasts derived from induced pluripotent stem cells

Fig. 2

Application of drug test platform by CBMC-iPSC-Fs. a Schematic for treatment of iPSC-Fs with TGF-β1 and pirfenidone. b Changes in thickness of 3D iPSC-F layer following TGF-β1 and pirfenidone treatment. Dotted lines and arrowheads indicate upper surface of 3D iPSC-F layer. Scale bars, 200 μm. c Effects of pirfenidone and TGF-β1 on skin thickness. d Quantification of total collagen content using hydroxyproline assay. e Gene expression of fibrotic markers COL1A1, COL1A2 COL3A1, and ACTA2 upon treatment with pirfenidone and TGF-β1. Graphs show mean with SEM of five independent samples. **p < 0.01, ***p < 0.001. GAPDH glyceraldehyde 3-phosphate dehydrogenase, TGF transforming growth factor

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