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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Establishment of a complex skin structure via layered co-culture of keratinocytes and fibroblasts derived from induced pluripotent stem cells

Fig. 3

Differentiation of iPSC-Ks and generation of iSO. a Schematic of iPSC-K differentiation process. b Morphology of iPSC-Ks on day 14. Scale bars, 100 μm. c–e Gene expression of (c) iPSC marker (OCT4), (d) neuroectoderm markers (PAX6 and SOX1), and (e) keratinocyte markers (Np63, KRT5, and KRT14). f Immunocytochemical analysis of Np63 (red) and KRT14 (green), together with DAPI staining (blue). Scale bars, 100 μm. Graphs show mean with SEM of five independent samples. **p < 0.01, ***p < 0.001. BMP4 bone morphogenetic protein 4, DAPI 4′,6-diamidino-2-phenylindole, DKSFM defined keratinocyte serum free medium, DMEM Dulbecco’s modified Eagle medium, EGF epidermal growth factor, GAPDH glyceraldehyde 3-phosphate dehydrogenase, iPSC induced pluripotent stem cell, iPSC-K iPSC-derived keratinocyte, KSFM keratinocyte serum free medium, n.s. not significant, RA retinoic acid

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