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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Establishment of a complex skin structure via layered co-culture of keratinocytes and fibroblasts derived from induced pluripotent stem cells

Fig. 4

Generation of CBMC-iPSC-derived skin organoid and humanized mice model. a Schematic of iSO generation process. b Histological analysis of iSO in vitro. Scale bars, 200 μm. c Transplantation of iSO into model mice. d Histological analysis of mouse skin and transplanted iSO. e Immunohistochemical analysis of mouse skin and transplanted iSO with OCT4, involucrin, loricrin, and KRT14. Scale bars, 200 μm. Graphs show mean with SEM of five independent samples. *p < 0.05, **p < 0.01, ***p < 0.001. FDM fibroblast differentiation medium, EP epithelial medium, H&E hematoxylin and eosin, iPSC induced pluripotent stem cell, iPSC-F iPSC-derived fibroblast, iPSC-K iPSC-derived keratinocyte, iSO induced pluripotent stem cell derived skin organoid, PSR Picrosirius Red

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