Fig. 1

Human bmMSCs, ucMSCs, kPSCs or CM-ucMSCs reduce glomerular podocyte and endothelial cell injury in ADR rats. a, b Quantification of podocyte number by morphometric analysis of WT1-positive podocytes in control and ADR rats receiving saline, bmMSCs, ucMSCs, kPSCs or CM-ucMSCs at 14 days (a) and 28 days (b). Data are mean ± SE. ***p < 0.001 vs control; °p < 0.05 and °°p < 0.01 vs ADR + saline. c Representative micrographs of renal sections from control and ADR rats receiving saline, bmMSCs, ucMSCs, kPSCs or CM-ucMSCs showing WT-1-positive podocytes (red) at 14 days. Scale bar 20 μm. d, e Quantification of capillary volume density (Vv) expressed as percentage of rat endothelial cell antigen-1 (RECA-1)-positive glomerular endothelial cells in control and ADR rats receiving saline, bmMSCs, ucMSCs, kPSCs or CM-ucMSCs at 14 days (d) and 28 days (e). Data are mean ± SE. **p < 0.01 and ***p < 0.001 vs control; °p < 0.05 and °°°p < 0.001 vs ADR + saline. f Representative images of glomerular endothelial cells stained with RECA-1 in renal sections of control and ADR rats receiving saline, bmMSCs, ucMSCs, kPSCs or CM-ucMSCs at 14 days. Scale bar 20 μm. ADR adriamycin, bmMSC bone marrow-derived mesenchymal stromal cell, CM-ucMSC conditioned medium obtained from umbilical cord-derived mesenchymal stromal cell, DAPI 4′,6-diamidino-2-phenylindole, kPSC kidney perivascular stromal cell