Fig. 2

Treatment with human bmMSCs, ucMSCs, kPSCs or CM-ucMSCs reduces synechiae formation. a Percentage of glomeruli affected by different degrees of synechiae (intercellular podocyte–PEC bridges) in control and ADR rats receiving saline, bmMSCs, ucMSCs, kPSCs or CM-ucMSCs at 14 days. To evaluate synechiae formation, each glomerulus was assigned a score between 0 (no lesion) and 4; values expressed as mean percentage ± SE. **p < 0.01 and ***p < 0.001 vs control; °p < 0.05, °°p < 0.01 and °°°p < 0.001 vs ADR + saline at the corresponding score. b Representative images of PECs and podocytes stained with claudin 1 (red) and nestin (green), respectively, in renal sections of control and ADR rats receiving saline, bmMSCs, ucMSCs, kPSCs or CM-ucMSCs at 14 days. Nuclei stained with DAPI (blue). Scale bar 20 μm. ADR adriamycin, bmMSC bone marrow-derived mesenchymal stromal cell, CM-ucMSC conditioned medium obtained from umbilical cord-derived mesenchymal stromal cell, DAPI 4′,6-diamidino-2-phenylindole, kPSC kidney perivascular stromal cell