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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Therapeutic potential of stromal cells of non-renal or renal origin in experimental chronic kidney disease

Fig. 5

Effect of human bmMSCs, ucMSCs or kPSCs on proliferation of activated PECs in co-culture system. a Schematic representation of experimental design with activated human PECs and stromal cells in co-culture using a transwell system. b, c Representative images and quantification of proliferating PECs positive for phospho H3-histone (P-H3) exposed to medium alone and angiotensin II (Ang II, 10− 7 M) (b) or albumin (alb, 10 mg/ml) (c) and co-cultured with bmMSCs, ucMSCs or kPSCs. PEC nuclei stained with DAPI. Data expressed as percentage of P-H3 positive PECs per total DAPI-positive cells/HPF. ***p < 0.001 vs PECs; °p < 0.05, °°p < 0.01 vs PECs + albumin; °°°p < 0.001 vs PECs + Ang II or albumin; ###p < 0.001 vs PECs + Ang II + kPSCs. d, e Representative images and quantification of Sestrin 2 (Sesn2) expression in PECs exposed to medium alone and Ang II (d) or alb (e) and co-cultured with bmMSCs, ucMSCs or kPSCs. PEC nuclei stained with DAPI (blue). Data expressed as MFI/cell. ***p < 0.001 vs PECs; °p < 0.05 vs PECs + Ang II; °°p < 0.01 vs PECs + Ang II or alb; °°°p < 0.001 vs PECs + alb; ##p < 0.01 vs PECs + alb + kPSCs. Data are mean ± SE. Scale bar 20 μm. bmMSC bone marrow-derived mesenchymal stromal cell, DAPI 4′,6-diamidino-2-phenylindole, HPF high-power field, kPSC kidney perivascular stromal cell, MFI mean fluorescence intensity, PEC parietal epithelial cell, ucMSC umbilical cord-derived mesenchymal stromal cell

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