Fig. 5
Effects of PJ34 on senescent EPC functionality after silencing sirtuin 1 (SIRT1). a–c SIRT1 expression was detected by Western blot after 48 h of SIRT1 short-hairpin RNA (Ad-sh-SIRT1) transfection. d Reactive oxygen species (ROS) production was assessed by DCFH-DA staining. e Intracellular nicotinamide adenine dinucleotide (NAD+) levels were measured. f Cell viability was evaluated via the CCK-8 assay. g, h Senescence was analyzed by senescence-associated beta-galactosidase (SA-β-gal) staining. i–l Tube formation ability was evaluated by Matrigel assay. m, n Migration ability was analyzed by Transwell assay. *P < 0.05, **P < 0.01, ***P < 0.001, versus the control; #P < 0.05, ##P < 0.01, ###P < 0.001, versus H2O2 treatment. NS not significant