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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Development and characterization of a polarized human endometrial cell epithelia in an air–liquid interface state

Fig. 1

Isolation and identification of endometrial epithelial cells. (A) The schematic showed the procedure of isolation of endometrial epithelial cells and generation of an air–liquid interface (ALI) culture. (B) Identification of endometrial epithelial cells. Cells grown with Rho-associated protein kinase (ROCK)-modified medium on collagen type I rat tail–coated dishes exhibited a capacity to form colonies, which expressed cell surface antigen epithelial cellular adhesion molecule (EpCam) but not CD13 as determined by an immunocytochemistry assay with hematoxylin counterstaining. (C) Immunofluorescent staining for Ki67 or stage-specific embryonic antigen-1 (SSEA-1) (green) revealed that a subset of primary human endometrial epithelial cells expressed Ki67 or SSEA-1. (D) Immunoblotting assay confirmed the expression of indicated proteins of interest in native human endometrial biopsy tissues and isolated cell cultures of passage 0–3. (E) Immunoblotting assay confirmed stem cell marker expression of Nanog, Oct3/4, Sox 2, p63, c-Myc, and CD117 (c-kit) in native human endometrial biopsy tissues and isolated cell cultures of passages 0–4. (E′) Semi-quantitative analysis of the fold changes of the expression of proteins in (E) accessed by a densitometric assay. Compared with passage 0 (P0) cells, *P <0.05; **P <0.01 (analysis of variance). Scale bars = 100 μm (C) and 25 μm (C′)

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