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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Development and characterization of a polarized human endometrial cell epithelia in an air–liquid interface state

Fig. 3

Whole mount immunofluorescent staining of endometrial epithelial cells cultured in an air–liquid interface (ALI) state and spheroid formation. The passage 1 (P1) endometrial epithelial cells were cultured in an ALI state for 2 weeks. (A) Human endometrial epithelial cells generated the spheroids by Matrigel sphere assay. (A′) A higher magnification of image (A). (B) Immunofluorescent staining for endometrial epithelial cell marker vascular endothelial-cadherin (VE-cadherin) (green). (B′) A higher magnification of image (B). (C) The co-expression of endometrial epithelial cell markers epithelial cellular adhesion molecule (EpCam) (green) and CD13 (red) was ascertained by immunofluorescence assay. Cell nuclei were counterstained with DAPI (blue). Arrows denote the co-localization of CD13 and EpCam staining (yellow). Scale bar = 25 μm

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