Fig. 4
The expression of endometrial epithelial marker in air–liquid interface (ALI) cultured cells. The passage 1 (P1) endometrial epithelial cells cultured in an ALI state for 2 weeks (A) or in a submerged state (B) were treated with estrogen for indicated time points, and the cell lysates were analyzed by immunoblotting assay against indicated antibodies against proteins of interest. (A, A′) Representative blots showed a significantly induced expression of indicated endometrial epithelial cell markers of ALI culture cells in response to estrogen (A). (A′) Semi-quantitative analysis of fold changes of the expression of indicated proteins in (A) accessed by a densitometric assay. (B, B′) Representative blots showed the expression of estrogen receptor (ER) and progesterone receptor (PR) of submerged cell cultures in response to estrogen (B). (B′) Semi-quantitative analysis of fold changes of the expression of proteins of interest in (B) accessed by a densitometric assay. Compared with cells cultured in the absence of estrogen, *P <0.05; **P <0.01; ***P <0.0001 (analysis of variance)