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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Inhibition of mTORC1 signaling protects kidney from irradiation-induced toxicity via accelerating recovery of renal stem-like cells

Fig. 3

mTORC1 signaling activated by irradiation in kidney tissues. a Expression of pS6 and pmTOR upregulated post irradiation in kidney tissues. Protein lysates prepared from kidney tissues at days 1, 3 and 7 (d1, d3, d7) after irradiation. Expression of pS6, S6, pmTOR and mTOR (left panel) detected by western blotting. GAPDH used as housekeeping control. Nonirradiated kidney tissues (CTL) used as controls. Expression of pS6, S6, pmTOR and mTOR (right panel) quantitated by ImageJ software and ratios of pS6/S6 and pmTOR/mTOR presented. b pS6 immunostaining in kidney tissues. Kidney tissues fixed in 4% paraformaldehyde after irradiation and pS6 immunostaining performed as indicated in Methods (left panel). Bar = 50 μm. Imaging software used to analyze integrated optical density (IOD) of pS6 (right panel). Arrows represent pS6-positive staining. c Expression of LC3-I and LC3-II in kidney tissues. Protein lysates prepared from kidney tissues at days 1, 3 and 7 after irradiation. Expression of LC3-I and LC3-II (left panel) detected by western blotting. GAPDH used as a housekeeping control. Nonirradiated kidney tissues (CTL) used as controls. Expression of LC3-I and LC3-II quantitated by ImageJ software and ratio of LC3-II and LC3-I expression presented (right panel). *p < 0.05 vs CTL; ***p < 0.001 vs CTL. CTL nonirradiated renal tissues, GAPDH glyceraldehyde 3-phosphate dehydrogenase, IHC immunohistochemistry, pmTOR pshosphorylated mammalian target of rapamycin, TBI total body irradiation

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