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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Inhibition of mTORC1 signaling protects kidney from irradiation-induced toxicity via accelerating recovery of renal stem-like cells

Fig. 6

Rapamycin treatment decreased cellular apoptosis induced by irradiation. C57BL/6 J mice exposed to 8.0 Gy of total body irradiation (TBI) and treated with vehicle (Veh) or rapamycin (Rap) starting at 6 h post exposure. Kidney tissues harvested at day 1 (d1) or day 7 (d7) post exposure. a, b Cellular apoptosis induced by irradiation. Representative pictures by TUNEL staining (a, arrowed). Ratios of apoptotic cells in each field counted and presented (b). c, d Cellular cleaved caspase-3 determined by immunostaining at day 1 after irradiation. Representative pictures by cleaved caspase-3 staining (c). Imaging software used to analyze integrated optical density (IOD) of cleaved caspase-3 (d). (e, f). BrdU incorporation assay in kidney tissues. BrdU (100 mg/kg) intraperitoneally injected 2 h before mice euthanized. BrdU-positive cells (e, arrowed) counted under light microscope and percentages of BrdU-positive cells in each field presented (f). Bar = 100 μm. *p < 0.05 vs Veh; ***p < 0.001 vs Veh. BrdU 5-bromo-2′-deoxyuridine, CTL nonirradiated renal tissues, N.D nondetected

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