Fig. 3

Effect of heat shock preconditioning on biological characteristics of MSCs. a Apoptosis rate of MSCs at 96 h, 120 h, 180 h and 240 h after HS at 42 °C for 1 h. Detected proliferative ability of MSCs with CCK-8. b Growth curve of HS MSCs and untreated MSCs. Flow cytometry to detect cell cycle by propidium iodide (PI) method at 48 h after HS. c Representative data for normal MSCs. d Representative data for HS MSCs. *P < 0.05, compared with normal group. HS heat shock, MSC mesenchymal stem cell