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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: A novel mutation of MSX1 in oligodontia inhibits odontogenesis of dental pulp stem cells via the ERK pathway

Fig. 3

Identification of human dental pulp stem cells (DPSCs) and nuclear localization of MSX1. a DPSCs transfected with wild-type (WT) and mutant (MT) MSX1 were positive for CD73 and CD29, and negative for CD34 and CD45. b DPSCs transfected with mutant MSX1 showed lower ki67+ cell numbers compared with cells transfected with wild-type MSX1. c Western blot analysis of cell total protein showed that the molecular weight of mutant MSX1 was remarkably lower than the wild-type. d Immunolocalization of intrinsic MSX1 and transfected flag-tagged MSX1 in DPSCs. Blue shows artificial color of nuclei by 4’,6’-diamidino-2-phenylindole (DAPI); red shows anti-flag tag staining. Intrinsic MSX1 localized exclusively in the nucleus. Wild-type MSX1 located exclusively in the nucleus as well, while mutant MSX1 was distributed over the entire cytoplasm. *P < 0.05. Data are expressed as the mean ± SD. Each experiment was repeated three times with n ≥ 3 samples per group

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Stem Cell Research & Therapy

ISSN: 1757-6512