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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: A novel mutation of MSX1 in oligodontia inhibits odontogenesis of dental pulp stem cells via the ERK pathway

Fig. 4

Effects of MSX1 on osteo/odontogenic differentiation of DPSCs. a MSX1 knockdown significantly decreased the capacity of osteo/odontogenic differentiation of hDPSCs compared with control. b, c Quantitative RT-PCR showed that dentin sialophosphoprotein (DSPP) and bone sialoprotein (BSP) was significantly downregulated after MSX1 knockdown compared with control. d Calcium nodule deposition of DPSCs after transfection was examined by Alizarin Red S staining. Mutant (MT) MSX1 showed decreased capacity of osteo/odontogenic differentiation compared with those ones transfected with wild-type (WT) MSX1. e, f Quantitative RT-PCR showed that DSPP and BSP was upregulated when hDPSCs were transfected with wild-type MSX1, while mutant MSX1 transfection showed lower expression levels compared with the wild type. *P < 0.05, **P < 0.01, ***P < 0.001. Data are expressed as the mean ± SD. Each experiment was repeated three times with n ≥ 3 samples per group. siRNA small interfering RNA

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