Fig. 2

Induction of induced pluripotent stem cells (iPSCs) to differentiate into otic progenitors. a iPSCs were induced to differentiate into otic progenitors and were stained for the presence of early otic markers PAX8 and PAX2, PAX8 and SOX2, and PAX8 and Nestin. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; blue). Scale bars = 20 μm. b Percentage of double-positive cells 12 days postinduction for PAX8 and PAX2, PAX8 and SOX2, and PAX8 and Nestin (n = 6, mean ± SD). c Reverse transcription-polymerase chain reaction (RT-PCR) analysis of gene expression of early otic markers SIX1, PAX2, PAX8, DLX5, and EYA1. The housekeeping gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal reference