Differentiation and transplantation of human induced pluripotent stem cell-derived otic epithelial progenitors in mouse cochlea

Chen, Jianling; Hong, Fanfan; Zhang, Cui; Li, Liang; Wang, Cuicui; Shi, Haosong; Fu, Yong; Wang, Jinfu

doi:10.1186/s13287-018-0967-1

Stem Cell Research & Therapy

Table 1 Primer sequences for reverse transcription-polymerase chain reaction

From: Differentiation and transplantation of human induced pluripotent stem cell-derived otic epithelial progenitors in mouse cochlea

Gene name	Forward primer (5’–3’)	Reverse primer (5’–3’)	Amplicon length	Annealing temperature
OCT4	GACAGGGGGAGGGGAGGAGCTAGG	CCTCCCTCCAACCAGTTGCCCCAAAC	144 bp	60 °C
SOX2	GGGAAATGGAGGGGTGCAAAAGAGG	TTGCGTGAGTGTGGA TGGGATTGGTG	151 bp	62.5 °C
KLF4	GAGGGAAGACCAGAATTCCCTTGA	AGAAC AA ACTCACCAAGCACCA	180 bp	60 °C
c-MYC	TGCACTGGAACTTACAA ACCCGA	TAA GCA GCT GCA AGG AGA GCC TTT	133 bp	60 °C
AFP	GAATGCTGCAAACTGACCACGCTGGAAC	TGGCATTCA AGAGGGTTTTCAGTTGGA	281 bp	62 °C
CK8	CCTGGA AGGGCTGACCGACGAGATCAA	CTTCCCAGCCAGGCCTGCAGCTCC	247 bp	65 °C
CK18	AGCTCAACGGGATCCTGCTG ACCTTG	CACTATCCGGCGG GTGGTGGTCTTTTG	233 bp	65 °C
MSX1	CGAGAGGACCCCGTGGATGCAGAG	GGCGGCCATCTTCAGCTTCT CAG	307 bp	63 °C
BRACHYURY	GCCCTCTCCCTCCCCTCCACGCACAG	CGGCGCCGTTGCTCACAGACCACAGG	274 bp	65 °C
PAX6	ACCCATTATCCAGATGTGTTTGCCCGAG	ATGGTGAAGCTGGGCATAGGCGG AG	317 bp	62 °C
SOX 1	CAACCAGGACCGGGCAAAC	CCTCGGACATGACCTTC AC	146 bp	58 °C
GAPDH	GAAGGTCGGAGTCAACGG	GGAAGATGGTGATGGGATT	496 bp	53 °C
SIX1	GACTCCGGTTTTCGCCTTTG	TAGTTTGAGCTCCTGGCGTG	878 bp	57 °C
PAX2	GAGCGAGTTCTCCGGCAAC	GTCAGACGGGGACGATGTG	225 bp	60 °C
PAX8	ACCCCCAAGGTGGTGGAGAAGA	CTCGAGGTGGTGCTGGCTGAAG	450 bp	62 °C
DLX5	TTCCAAGCTCCGTTCCAGAC	GTAATGCGGCCAGCTGAAAG	409 bp	57 °C
EYA1	TCAGATGCTATCTGCCGCTG	GTGCCATTGGGAGTCATGGA	595 bp	57 °C
ATOH1	GCCGCCCAGTATTTGCTACA	GCTAGCCGTCTCTGCTTCTG	260 bp	57 °C
BRN3C	TGCAAGAACCCAAATTCTCC	GAGCTCTGGCTTGCTGTTCT	758 bp	55 °C
MYO7A	CACATCTTTGCCATTGCTGAC	AGAAGAGAACCTCACAGGCAT	649 bp	55 °C
ESPIN	CAGGCATGTCCTCACCCAAT	CGTGGCGGAGTTTGTTCTTG	384 bp	55 °C
NEUROD1	GCCCCAGGGTTATGAGACTATCACT	CCGACAGAGCCCAGATGTAGTTCTT	523 bp	59 °C
POU4F1	AACAGCAAGCAGCCTCACTT	TTCATCGTGTGGTACGTGGC	245 bp	55 °C
NTRK2	AATTGGGTTGGAGCAGGAGC	GGGTCCATGCCACCTTATCC	522 bp	56 °C
ISLET1	CAACAAACAAAACGCAAAAC	AAGTCAAACACAATCCCGA	542 bp	50 °C

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ISSN: 1757-6512

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