Skip to main content
Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Development and validation of broad-spectrum magnetic particle labelling processes for cell therapy manufacturing

Fig. 1

Microparticle-based labelling of adherent cultures incubated for 24 h in medium containing 1000 nm MPs (10 μg/μl). af Flow cytometry measurement of labelled (pink) and unlabelled (blue) cell populations analysed 24 h post incubation: a HeLa (cervical cancer stem cell line), b pMSC (primary mesenchymal stem cells), c HOS (human osteosarcoma stem cell line), d mESC (mouse embryonic stem cells), e SHSY5Y (human neuroblastoma derived stem cell line), f Caco-2 (human epithelial colorectal adenocarcinoma cells). Representative data shown, n = 3. gi Fluorescence microscopy observation of neuroprogenitors (ReN, g), mesenchymal stem cells (MSC, h), and iPS-derived cardiomyocytes (CMC, i) incubated overnight with MPs (gold), stained with Phalloidin (green) and Hoechst 33342 (blue)

Back to article page