Skip to main content

Advertisement

Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Senescence of bone marrow-derived mesenchymal stem cells from patients with idiopathic pulmonary fibrosis

Fig. 6

Mitochondria in B-MSCs from IPF patients have lower OCR and ECAR compared with aged-matched controls. Real-time measurements (mean ± SEM, n = 4 with technical triplicates) of the mitochondrial oxygen consumption rate (OCR; pmol O2/min) and extracellular acidification rate (ECAR; mpH/min) of B-MSC were measured under basal conditions and in response to the indicated mitochondrial inhibitors. OCR (a) and ECAR (b) in idiopathic pulmonary fibrosis (IPF) B-MSCs (continuous line) is significantly lower compared with B-MSC from age-matched controls (Old; dashed line). The basal relationship between OCR and ECAR (c) of IPF B-MSCs was lower compared with controls. Adenosine triphosphate (ATP) content in IPF B-MSCs (d) was measured by bioluminescence assay. Total ATP content was shown to be nonsignificantly lower in IPF B-MSCs compared with age-matched controls. To evaluate the effects of transforming growth factor (TGF)-β stimulation on B-MSCs from IPF and control patients, B-MSCs were stimulated with human recombinant activated TGF-β1 (5 ng/ml). Real-time measurements (mean ± SEM, n = 4 with technical triplicates) of the mitochondrial OCR and ECAR of B-MSCs were measured under basal conditions and in response to the indicated mitochondrial inhibitors. OCR in basal (continuous line) or TGF-β1-stimulated (dashed line) B-MSCs are shown for control (e, g) and IPF (f, h) patients; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. 2-DG 2-deoxy-d-glucose, DMEM Dulbecco’s modified Eagle’s medium, FCCP carbonyl cyanide p-trifluoromethoxyphenylhydrazone, Oligo. oligomycin, Rote. rotenone

Back to article page