Fig. 8

Stimulation of human lung fibroblasts (HLF) with bone marrow-derived mesenchymal stem cell (B-MSC) conditioned media (CM) from idiopathic pulmonary fibrosis (IPF) patients recapitulates senescence and fibrotic phenotypes in old HLF. HLF from a 65-year-old male were stimulated with CM from B-MSC of controls (Old; 73 ± 4 years of age) and IPF patients (72 ± 5 years of age) for 2 days. a HLF were subjected to SA-β-gal staining and percent of senescent cells were quantified (b). c mRNA expression levels in stimulated HLF were determined by qRT-PCR for senescent markers (p21 and p53) and d profibrotic SASPs (fibronectin (FN)1, collagen (Col)1, ACTA1, and Col3); mean ± SEM; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. Treatment with nonconditioned media does not increase transcript levels in HLF (data not shown). FC fold-change