Fig. 6

Histological assessments of DS (a) and POCs-DS (b) transplanted grafts. a H&E staining revealed the presence of host cells and suitable neovascularization (black arrows). Scale bar: 100 μm. b A high number of viable ovarian cells population in the POCs-seeded scaffolds. POCs were found in clusters inside the scaffolds and several primordial or primary follicle-like structures were identified (black arrow and c). Scale bar: 100 μm. c Lightly stained round oocytes were surrounded by different numbers of granulosa cells in close contact with each other. Scale bar: 20 μm. DS decellularized scaffolds, POCs primary ovarian cells, POCs-DS primary ovarian cells-seeded decellularized scaffolds