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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Mesenchymal stem cells alleviate experimental autoimmune cholangitis through immunosuppression and cytoprotective function mediated by galectin-9

Fig. 3

Galectin-9 (Gal-9) is highly expressed in umbilical cord–derived mesenchymal stem cells (UC-MSCs). a Total mRNA of UC-MSCs was extracted after activation with or without interferon-gamma (IFN-γ) and analyzed by real-time polymerase chain reaction for the expression of Gal-1, Gal-3, and Gal-9. Bars represent the mean ± standard error of the mean (SEM); n = 4 per group. b, c UC-MSCs were treated with or without IFN-γ and analyzed by Western blot and enzyme-linked immunosorbent assay for the expression of Gal-9. Bars represent the mean ± SEM; n = 3 per group. d The fluorescence microscope was employed to detect green fluorescent protein (GFP)-positive UC-MSCs in the livers of primary biliary cholangitis mice. Colocalization (white arrow) of the GFP (green) with Gal-9 (red) staining indicated that UC-MSCs expressed Gal-9. Scale bar = 50 μm. Abbreviation: DAPI 4′,6-diamidino-2-phenylindole

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Stem Cell Research & Therapy

ISSN: 1757-6512