Table 1 Primers for real-time PCR
Genes | Primers | |
|---|---|---|
Forward (5‘-3’) | Reverse (5‘-3’) | |
Human GAPDH | GCACCGTCAAGGCTGAGAAC | TGGTGAAGACGCCAGTGGA |
Human Galectin-1 | AGGTTGTTGCTGTCTTTGCC | CAAACCTGGAGAGTGCCTTC |
Human Galectin-3 | TGCAACCTTGAAGTGGTCAG | GGGGAAGGGAAGAAAGACAG |
Human Galectin-9 | ATAGTCCCAGAAAAGGGGACA | TCCTAGTGGGTGTGAAAGGC |
Human CXCL10 | GCTGATGCAGGTACAGCGT | CACCATGAATCAAACTGCGA |
Human CCL5 | TGTACTCCCGAACCCATTTC | TACACCAGTGGCAAGTGCTC |
Human CCL20 | CGTGTGAAGCCCACAATAAA | GTGCTGCTACTCCACCTCTG |
Human CX3CL1 | ACGTGATGTTGCATTTCGTC | CCGATATCTCTGTCGTGGCT |
Mouse GAPDH | TTGATGGCAACAATCTCCAC | CGTCCCGTAGACAAAATGGT |
Mouse IFN-γ | ATGAACGCTACACACTGCATC | CCATCCTTTTGCCAGTTCCTC |
Mouse IL-17A | TTTAACTCCCTTGGCGCAAAA | CTTTCCCTCCGCATTGACAC |
Mouse IL-12 | GCTTCTCCCACAGGAGGTTT | CTAGACAAGGGCATGCTGGT |
Mouse IL-23 | GCTCCCCTTTGAAGATGTCA | GACCCACAAGGACTCAAGGA |
Mouse T-bet | ATCCTGTAATGGCTTGTGGG | TCAACCAGCACCAGACAGAG |
Mouse RORγt | GGTGATAACCCCGTAGTGGA | CTGCAAAGAAGACCCACACC |
Mouse CXCL10 | CCTATGGCCCTCATTCTCAC | CTCATCCTGCTGGGTCTGAG |
Mouse CCL5 | CCACTTCTTCTCTGGGTTGG | GTGCCCACGTCAAGGAGTAT |
Mouse CCL20 | TGTACGAGAGGCAACAGTCG | TCTGCTCTTCCTTGCTTTGG |
Mouse CX3CL1 | TGGGATTCGTGAGGTCATCT | CGCGTTCTTCCATTTGTGTA |