Fig. 5

Detection of allogeneic BMSC in lungs and peripheral blood of BLM-treated mice. After male BMSC treatment in the BLM-treated female mice, peripheral blood and lung samples were collected at different time points. a-b Representative PCR analysis of SRY gene from (a) lung and (b) peripheral blood from BLM-treated female mice transplanted with male BMSC is shown. For PCR, peripheral blood and lung samples collected from female and male mice were used as negative and positive control. c Lung specimens and (d) peripheral blood were collected and the mRNA expression of SRY gene was assessed by quantitative RT-PCR (n = 3). The results shown are normalized to GAPDH