Fig. 2

HUVEC migration groups. Group 1: HUVECs cultured alone (a), or with 100 mM glucose (b), or with 100 mM glucose and 25% conditioned medium obtained from unstimulated pMSC culture (CMpMSC) (c) in upper chamber of CIM migration plate, while HUVEC medium with 30% FBS added to lower chamber. Group 2: HUVECs seeded in HUVEC serum-free medium in upper chamber of CIM migration plate while HUVEC medium with 30% FBS (a), or with 100 mM glucose (b), or with 100 mM glucose and 20% CMpMSC (b) added to lower chamber of migration plate. Group 3: HUVEC cultured alone (a), or with 100 mM glucose (b), or with 100 mM glucose and 25% CMpMSC (c), or with pMSCs at ratio of 1 HUVEC:1 pMSC in intercellular direct contact experiment (ICpMSC) (d). Pretreated HUVECs seeded in HUVEC serum-free medium in upper chamber of CIM migration plate while HUVEC culture medium containing 30% FBS added to lower chambers. CMpMSC conditioned medium of unstimulated pMSCs, FBS fetal bovine serum, HUVEC human umbilical vein endothelial cell, pMSC placental mesenchymal stem cell