Fig. 4From: Human chorionic villous mesenchymal stem/stromal cells protect endothelial cells from injury induced by high level of glucoseHUVEC proliferation in response to glucose in presence of pMSCs, or after removing glucose and pMSCs, examined after 24, 48, and 72 h in MTS assay. In response to conditioned medium (CMpMSC), pMSCs had no significant effect on HUVEC proliferation in presence of glucose after 24 h as compared to untreated or glucose-treated HUVECs (a). CMpMSC significantly increased HUVEC proliferation in presence of glucose after 48 h (b) and 72 h (c), as compared to glucose-treated but not untreated HUVECs. Cell–cell contact assay showed that, compared to glucose-untreated or treated HUVECs, pMSCs significantly increased HUVEC proliferation in presence of glucose after 24 h (a) and 48 h (b), while after 72 h (c) pMSCs significantly increased HUVEC proliferation in presence of glucose, as compared to glucose-treated but not untreated HUVECs. HUVEC proliferation after removing effects of glucose and pMSCs. HUVECs initially cultured with 100 mM glucose (100(pre)) in presence of different treatments of pMSCs (CMpMSC(PreCM + 100) and ICpMSC(PreCM + 100)) for 72 h, and then used in proliferation assay using xCELLigence real-time cell analyser. After 24 and 48 h (d, e), proliferation of HUVECs pretreated with glucose alone (100(pre)), or with CMpMSC (PreCM + 100) or ICpMSC (PreCM + 100), did not significantly change as compared to glucose-untreated HUVEC (p > 0.05). As compared to glucose-treated HUVECs (100(pre)), proliferation of HUVECs pretreated with glucose and CMpMSC (PreCM + 100), or glucose and ICpMSC (PreIC + 100), did not significantly change after 24 and 48 h (p > 0.05) (d, e). In contrast, proliferation of HUVECs pretreated with 100 mM glucose alone (100(pre)), or with CMpMSC (PreCM + 100), significantly reduced after 72 h as compared to glucose-untreated HUVEC (f). When compared with glucose-treated HUVECs (100(pre)), proliferation of HUVECs pretreated with glucose and CMpMSC (PreCM + 100) did not significantly change after 72 h of culture. In contrast, proliferation of HUVECs pretreated with glucose and ICpMSC (PreIC + 100) increased significantly after 72 h of culture as compared to glucose-treated but not untreated HUVECs (f). Each experiment performed in triplicate using HUVECs (passage 3–5) and pMSCs (passage 2) from five independent umbilical cord tissues and placentae, respectively. *P value is significant p < 0.05. Bars represent standard errors. pMSC placental mesenchymal stem cellBack to article page