Fig. 7

HUVEC permeability under effects of glucose and pMSCs examined by adding monocytes to monolayer of HUVECs and assessing invasion of monocytes through HUVEC monolayer by xCELLigence real-time system. Increased invasion defined as reduction in cell index due to infiltration of HUVEC monolayer by monocytes, causing detachment of HUVECs, while increased cell index defines reduction in cell invasion. In presence of 100 mM/ml glucose (100(in)), monocyte invasion of HUVEC monolayer significantly increased after 10 h as compared to glucose-untreated HUVECs (a). After 10 h and as compared to glucose-untreated HUVECs, monocyte invasion in presence of glucose and CMpMSC (100 + CM(in)) significantly reduced but not significantly changed as compared to glucose-untreated HUVECs (a). Monocyte invasion through monolayer of HUVECs pretreated with glucose alone (100(pre)), or with glucose and CMpMSC (100 + CM(pre)), not significantly changed after 10 h as compared to glucose-untreated HUVECs, while addition of ICpMSC (100 + IC(pre)) significantly reduced monocyte invasion as compared to glucose-treated (100(pre)) or untreated HUVECs (b). Each experiment performed in triplicate using HUVECs (passage 3–5) and pMSCs (passage 2) from five independent umbilical cord tissues and placentae, respectively. *P value is significant < 0.05. Bars represent standard errors