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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Development of a novel and economical agar-based non-adherent three-dimensional culture method for enrichment of cancer stem-like cells

Fig. 5

Detection and visualization of prostate cancer stem-like cells (PCSCs) present in three-dimensional (3D) cultured prostatospheres by Sox2/Oct4 response element six tandem repeats (SORE6) reporter system. a Fluorescent (upper) and phase contrast (lower) images of DU145-SORE6 cell–derived prostatospheres shown at low (left; bars: 100 μm) and high (right; bars: 20 μm) magnification. b z-Stack visualization of individual DU145-SORE6–derived prostatospheres (animation shown in Additional file 1). Results showed that the DU145-SORE6–derived prostatospheres contained SORE6+ cells, suggesting that the 3D cultured prostatospheres were enriched of PCSCs. c Reverse transcription quantitative polymerase chain reaction analysis of expression levels of OCT4, SOX2, and NANOG in flow cytometry–sorted DU145-SORE6 cells. *P <0.05 SORE6+ versus SORE6− cells

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Stem Cell Research & Therapy

ISSN: 1757-6512