Table 1 Nucleotide sequences of primers used for quantitative polymerase chain reaction analysis
Gene name | Alias | Forward (5′-3′) | Reverse (5′-3′) |
|---|---|---|---|
CD24 | CD24 | CTCCTACCCACGCAGATTTATTC | TGGTGGCATTAGTTGGATTTGG |
CD44 | CD44 | CAGCACCATTTCAACCACAC | GTTGCCAAACCACTGTTCCT |
PROM1 | CD133 | AAACAGTTTGCCCCCAGGAA | ACAATCCATTCCCTGTGCGT |
KRT5 | CK5 | AGGAGTTGGACCAGTCAACAT | TGGAGTAGTAGCTTCCACTGC |
KRT14 | CK14 | TGAGCCGCATTCTGAACGAG | GATGACTGCGATCCAGAGGA |
NANOG | NANOG | TTTGTGGGCCTGAAGAAAACT | AGGGCTGTCCTGAATAAGCAG |
BMI1 | BMI1 | GCTGCCAATGGCTCTAATGAA | TGCTGGGCATCGTAAGTATCTT |
POU5F1 | OCT4 | GACAACAATGAAAATCTTCAGGAGA | CTGGCGCCGGTTACAGAACCA |
SOX2 | SOX2 | GCCGAGTGGAAACTTTTGTCG | GGCAGCGTGTACTTATCCTTCT |
CTNNB1 | β-catenin | TTGTGCGGCGCCATTTTAAG | TCCTCAGACCTTCCTCCGTC |
AR | AR | CGGAAGCTGAAGAAACTTGG | ATGGCTTCCAGGACATTCAG |
KLK3 | PSA | TTGTCTTCCTCACCCTGTCC | TCACGCTTTTGTTCCTGATG |
KLK2 | Kallikrein 2 | CTGCCCATTGCCTAAAGAAG | GCTCACACACTGAAGACTCCTG |
ACTB | β-actin | ATGGATGATGATATCGCCGCG | CTCCATGTCGTCCCAGTTGGT |