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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Mesenchymal stem cells recruited by castration-induced inflammation activation accelerate prostate cancer hormone resistance via chemokine ligand 5 secretion

Fig. 5

NAC alleviates PCa recruitment of MSCs via oxidative stress suppression. a Intracellular ROS levels estimated in PCa cells with AD after 5 mM NAC added to media. b PCa cells on MSC migration demonstrated after NAC added in AD. c Western blot analysis of NF-κB activation in PCa cells after adding NAC in AD. d RT-PCR analysis applied to determine expression of IL-1β in PCa cells after adding NAC. e In LNCaP xenograft mouse model, NAC (100 mg/kg) administered intraperitoneally daily after castration. Tumors measured by calipers, then volume calculated. f 4-HNE adduct levels determined in tumor homogenates when tumors removed at 4th week. g Influence of NAC on migration of MSCs to tumor sites when tumor-bearing mice castrated. h Western blot analysis of GFP expression in tumor tissues. i Influence of NAC on expression of IL-1β in tumor tissues when mice castrated. *p < 0.05, **p < 0.01. AD androgen deprivation, GFP green fluorescent protein, IL interleukin, NAC N-acetylcysteine, ROS reactive oxygen species, MSC mesenchymal stem cell

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