Fig. 1

Study schedule and preparation of cells for transplantation. a Study schedule. UPP, urethral pressure profile; Tx, transplantation; * - in the PBS group one animal was lost during the experiment; b proliferating caprine muscle-derived cells (MDCs); c proliferating caprine bone marrow mesenchymal stem cells (MSCs); d MSCs differentiated into adipocytes - Oil Red O staining; e MSCs differentiated into osteocytes - Alizarin Red staining; f MSCs differentiated into chondrocytes - Safranin O staining; g undifferentiated MDCs immunostained for desmin (red fluorescence); h MDCs after myogenic differentiation - multinucleated myotubes can be observed (desmin is red and cell nuclei are blue); i MDCs labeled with PKH67 - a green fluorophore; j MDCs labeled with DID - a far red fluorophore; k mean (± SEM) number of cells (in total or only living cells) used for transplantation in the experimental groups. No statistical differences were noted between groups