Fig. 6
Myogenic differentiation of transplanted cells within the urethral wall (28th day time point). Images of cross-sections after immunohistochemical staining. 1,1′-Dioctadecyl-3,3,3,-tetrametlylindodicarbocyanine-5,5-disulfonic acid (DID)-labeled transplanted cells are red, AF488-labeled desmin is green, 4',6-diamidino-2-phenylindole (DAPI)-labeled nuclei are blue. (A) Urethra from the mesenchymal stem/stromal cells (MSC) group; (B) urethra from the muscle-derived cells (MDC) group; (C) urethra from the co-transplanted group. Scale bar 200 μm; D, E, F images corresponding to the area marked up on the neighboring picture (with merged and separated fluorescent channels). Arrows indicate areas where DID-labeled cells are present within muscle fibers; arrowheads indicate areas where DID-labeled cells remain undifferentiated; pointed arrows (MDC-MSC) indicate DIDpos/desminpos elongated structures with centrally located nulcei (de novo created young muscle fibers)