Fig. 3

BM-MSCs enhance demethylation of TSDR in coculture system. CD4⁺ effector T cells and DCs isolated and cocultured with MSCs under four conditions as described in Methods. Genomic DNA extracted from MSC-cultured T cells after 72 h, and TSDR methylation assessed by bisulfite conversion and HRM as described in Methods. Allogeneic MLR performed; CD4⁺CD25⁻ effector T cells isolated 5 days after MLR used as negative control, and TGF-β-induced Tregs used as positive control. DC dendritic cell, iTreg induced Treg, LPS lipopolysaccharide, MSC mesenchymal stem cell, MSC-DC MSC-treated DC, MLR mixed lymphocyte reaction, TC allogeneic CD4⁺CD25⁻ T cells