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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Induction of CD4+CD25+FOXP3+ regulatory T cells by mesenchymal stem cells is associated with modulation of ubiquitination factors and TSDR demethylation

Fig. 4

MSCs reduce proinflammatory cytokine production and increase IL-2 and IL-10 production. CD4+ effector T cells and DCs isolated and cocultured with MSCs under four conditions as described in Methods. Supernatant collected, and levels of TNF-α, INF-γ, IL-4, IL-5, IL-17, IL-6, IL-21, IL-2, and IL-10 measured after 72 h and 5 days of coculturing by flow cytometry and compared with negative control. Data presented as mean ± SEM; n = 4 independent experiments. Significant results: *p < 0.05; **p < 0.01; ***p < 0.001. d days, DC dendritic cell, IL interleukin, INF interferon, iTreg induced Treg, LPS lipopolysaccharide, MSC mesenchymal stem cell, MSC-DC MSC-treated DC, MLR mixed lymphocyte reaction, TC allogeneic CD4+CD25 T cells. TNF tumor necrosis factor

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