Fig. 5

In-vivo administration of BM-MSCs prolongs skin allograft survival. BALB/c mice grafted with full-thickness allogeneic back skin from C57BL/6 mice and treated with PBS, MSCs, or cyclosporin as described in Methods. a Survival graph of transplanted mice showed median survival time (MST) of MSC-treated mice is 17 days (n = 5), of cyclosporin-treated animals is 21 days (n = 5), and of PBS-treated animals (n = 5) is approximately 11 days. b For in-vivo suppression assay, T cells isolated from skin-transplanted mice and added to T cells that were stimulated with allogeneic DCs. After 48 h, BrdU kit used to measure proliferation of CD4⁺ T cells, and compared with that of cells from PBS-treated mice. Data presented as mean ± SEM; n = 5 in each group. Significant results: *p < 0.05; ***p < 0.001. CsA cyclosporin A, MSC mesenchymal stem cell, PBS phosphate buffered saline