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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Induction of CD4+CD25+FOXP3+ regulatory T cells by mesenchymal stem cells is associated with modulation of ubiquitination factors and TSDR demethylation

Fig. 6

Infusion of BM-MSCs delays skin graft rejection via increased FOXP3 expression and modification of ubiquitination gene expression in iTregs. BALB/c mice grafted with full-thickness allogeneic back skin from C57BL/6 mice and treated with MSCs, cyclosporin, or PBS as described in Methods. Mice sacrificed on days 5, 10, or 15, and total mRNA extracted from splenic CD4+ T cells. Expression of TRAF6, GRAIL, USP7, UBC13, and STUB1 mRNA assessed by quantitative RT-PCR. mRNA samples normalized to expression of endogenous housekeeping gene (GAPDH) and compared with PBS-treated group. Data presented as mean ± SEM; n = 5 in each group. Significant results: *p < 0.05; ***p < 0.001. CsA cyclosporin A, MSC mesenchymal stem cell

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